PICSI – Physiological ICSI

Sperm Selection in In Vitro Fertilization (IVF)

Also known as physiological ICSI, PICSI (Physiological Intracytoplasmic Sperm Injection) is a technique used in In Vitro Fertilization, which allows sperm to be selected with a greater degree of maturity. Unlike traditional ICSI, in which sperm selection is performed by observation by the embryologist, the PICSI technique aims to find a less subjective system, selecting the more mature sperm using a molecule similar to the one surrounding the oocyte natural form.


1. PICSI technique procedure

In an In Vitro Fertilization (IVF) the best gametes are selected for fertilization and, the resulting embryo, transferred to the mother’s womb. IVF follows the same procedure with both techniques – ICSI and PICSI – except for the selection part of the male gamete. After semen collection and sperm training, a special plate is used to deposit them. The sperm are deposited on a plate containing a few drops of a synthetic material very similar to hyaluronic acid – the main substance of the cells surrounding the ovule. Good quality sperm will adhere to these drops and the embryologist can identify and select them easily. Once the best quality sperm is selected, it is aspirated with the microinjection pipette.

The selection process makes the sperm chosen through the PICSI technique higher quality than those selected by observation.

2. In which patients is the PICSI technique indicated?

The PICSI technique is indicated for those couples in which the male has poor sperm quality, or who have previously undergone IVF with the ICSI technique and have not achieved the desired results.


Men with high level of DNA fragmentation in their sperm.


Cases of repeated abortion in which the cause is male or unknown.


When there is low concentration and poor semen morphology.


Cases in which there have been previous cycles with poor embryonic quality.

3. Advantages of the PICSI technique

The PICSI technique becomes a very effective technique by selecting the most mature sperm, with less damage to their DNA and presenting a lower risk in chromosomal abnormalities. Unlike the ICSI, in which an observation method (subjective) is used, the PICSI adds an objective method to the ICSI, which allows increasing the probability of pregnancy and reducing the possibility of abortion.


Get in touch
with us

Life is the art of encounter. We want to be part of your path. We aim you to have the best quality of life.



Grand Hotel Djibloho, Djibloho (Guinea Ecuatorial)