Fluorescence in situ hybridization (FISH) in Human Sperm

Fluorescent in situ hybridization (FISH) is a technique that is performed on the semen sample and that consists of applying specific DNA probes for certain chromosomal regions. Chromosomes 13, 18, 21, X and Y are usually evaluated and can be extended to other chromosomes.
With this technique, the aim is to verify that the chromosomal endowment (number of copies of each chromosome) is correct or that there is any type of alteration that could ultimately affect the chromosomal endowment of the generated embryos.

The indications for performing this technique are when there have been repeated abortions or implantation failures and in men with poor seminal quality or who have an altered karyotype.


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